Journal of Experimental Medicine, Vol 142, 1403-1415, Copyright © 1975 by Rockefeller University Press

ARTICLES

Fc-receptors, Ia-antigens, and immunoglobulin on normal and activated mouse T lymphocytes

PH Krammer, L Hudson and J Sprent

Using antibody coated bovine erythrocytes we were unable to demonstrate Fc-receptors on either thymus cells or T cells prepared from lymph node cell suspensions by anti-Ig column filtration. However, if parental thymus or lymph node T cells were transferred to X-irradiated F1 hybrids, activated donor T cells recovered from the recipient's spleen (ATC-spleen) were shown to express Fc-receptors. Fc-receptors were also demonstrable on ATC-spleen prepared between strain combinations differing at the M-locus. In marked contrast, Fc-receptors were not detected on ATC recovered from thoracic duct lymph (T.TDL). This applied to (a) H-2-activated T.TDL derived from normal thymus cells, (b) H-2-activated T.TDL derived from thymus cells depleted of B cells, and (c) M-locus-activated T.TDL. Of these three populations, surface Ig (of B cell origin) was detected on a large proportion of the first but not on the second and third populations. Thus, the failure to detect Fc- receptors on any of these populations could not be attributed to blocking by adsorbed surface Ig. In addition, various T-cell populations were examined by a microcytotoxicity assay for the presence of cell surface Ia-antigens. 5--10% of the thymus cells, 20--30% of cortisone-resistant thymus cells, 60--70% of lymph node cells, and 60-- 80% of ATC-spleen and T.TDL showed Ia.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Hudrisier, D., Clemenceau, B., Balor, S., Daubeuf, S., Magdeleine, E., Daeron, M., Bruhns, P., Vie, H. (2009). Ligand Binding but Undetected Functional Response of FcR after Their Capture by T Cells via Trogocytosis. J. Immunol. 183: 6102-6113 [Abstract] [Full Text]  
• Patel, D. M., Arnold, P. Y., White, G. A., Nardella, J. P., Mannie, M. D. (1999). Class II MHC/Peptide Complexes Are Released from APC and Are Acquired by T Cell Responders During Specific Antigen Recognition. J. Immunol. 163: 5201-5210 [Abstract] [Full Text]  
• CRYSTAL, R. G., ROBERTS, W. C., HUNNINGHAKE, G. W., GADEK, J. E., FULMER, J. D., LINE, B. R. (1981). Pulmonary Sarcoidosis: A Disease Characterized and Perpetuated by Activated Lung T-Lymphocytes. ANN INTERN MED 94: 73-94 [Abstract]  
• Grey, H. M., Anderson, C. L., Heusser, C. H., Borthistle, B. K., Von Eschen, K. B., Chiller, J. M. (1977). Structural and Functional Heterogeneity of Fc Receptors. Cold Spring Harb Symp Quant Biol 41: 315-321 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS