Journal of Experimental Medicine, Vol 142, 974-988, Copyright © 1975 by Rockefeller University Press
Immunological and physiological characteristics of the rapid immune hemolysis of neuraminidase-treated sheep red cells produced by fresh guinea pig serum
PK Lauf
The rapid hemolysis by fresh guinea pig serum known to occur with
neuraminidase-treated sheep red cells has been investigated with respect to
the immunological and physiological properties of the lytic process. The
following observations were made: (a) The susceptibility to hemolysis is
directly proportional to the amounts of neuraminic acid enzymatically
released from the cell surface. Complement lysis is mediated through
binding of an IgM antibody protein to membranes of neuraminidase-treated
cells. (b) Hemolysis is relatively temperature- independent above about 28
degrees C but below which a decrease in the hemolysis rate occurs.
Arrhenius activation energies above and below the transition temperature
were therefore found to be different. (c) Colloid osmotic swelling of
neuraminidase-treated high potassium sheep red cells with a chloride ion
concentration ratio near unity suspended in high potassium medium could not
be prevented by sucrose. Hence, colloid osmotic swelling before lysis must
be due to the entrance of sucrose and water since sucrose was the only
external solute not at equilibrium. (d) From the rate of swelling and the
apparent flux of sucrose under nonsteady state conditions an experimental
permeability coefficient (P) for sucrose of 3-10(-8) cm-s-1 was computed.
Comparison with a theoretical P of 4-10(-6) cm-s-1 for sucrose freely
permeating through a single, hypothetical membrane lesion per cell of 60 A
effective diameter indicates a membrane lesion which permits the passage of
solutes larger than cations, but clearly constrains the free diffusion of
sucrose.
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