Journal of Experimental Medicine, Vol 141, 775-787, Copyright © 1975 by Rockefeller University Press
Study of the cells proliferating in parent versus F hybrid mixed lymphocyte culture
PF Piguet, HK Dewey and P Vassalli
Caryotypic analysis of the cells dividing in mouse parent-hybrid MLC showed
an F1 hybrid cell proliferation, which varied depending upon the source of
lymphoid cells used: strong in spleen MLC (sometimes equal to that of the
parental cells), less marked in lymph node cell MLC, and most often absent
in MLC between cortisone-resistant (CR) thymocytes. MLC between parental
spleen cells and F1 CR thymocytes showed, however, that in certain
conditions of culture F thymocytes can also proliferate. Using parental or
F1 spleen cells lacking T lymphocytes, it was found that F1 cell
proliferation is entirely dependent upon the presence of parental T cells,
but does not require the presence of T lymphocytes among the F1 cells.
Immunofluorescence analysis of the blasts observed in one-way MLC showed
that about 70% of the parental blasts were T blasts, and 25%B blasts
(containing a high proportion of plasmablasts); among the F1 blasts, there
was also the same percentage of B blasts and plasmablasts, but many of the
T blasts bore only small amounts of T-cell antigen (MTLA), and there was
also about 20%of unstained blasts, possibly T blasts bearing MTLA in
amounts undetectable by immunofluorescence. The possibility is discussed
that the F1 responding T cells belong to a subpopulation performing a
suppressive function; MLC lacking F1 T cells showed increased [3H]
thymidine incorporation. The proliferation and differentiation of parental
and F1 B cells may result mainly from an unspecific, "polyclonal"
triggering.
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