Journal of Experimental Medicine, Vol 141, 360-373, Copyright © 1975 by Rockefeller University Press
Evidence for a B-cell -like helper function in mixed lymphocyte culture between immunocompetent thymus cells
JW Kyminski and RT Smith
The immunocompetent subpopulation by mouse thymus cell (TH-2) was isolated
by buoyant density centrifugation and by hydrocortisone pretreatment. TH-2
cells undergo a proliferative one-way or two-way mixed lymphocyte culture
(MLC) response only when cultured with allogeneic or congenic peripheral
lymphoid cells. However, mixtures of allogeneic TH-2 cells alone do not
proliferate in either one-way or two- way MLC reactions. Such MLC mixtures
are proliferative only if mitomycin-blocked peripheral lymphoid cells are
also present in the mixture. The peripheral helper cell has been found to
be of low net density, non-adherent, insensitive to anti-thy-1 serum
cytotoxicity, but sensitive to the cytotoxic effets of anti-immunoglobulin
serume plus complement. The helper effect does not depend on proliferation
nor does it appear to involve demonstrable soluble mediators. The nature of
failure of MLC between TH-2 subpopulations appears to be dependent on the
exppression of some product of the K, I regions of the H-2 locus. Possible
mechanisms by which a B-cell-like helper cell triggers TH-2 proliferation
are discussed terms of the present knowledge of specific alloantigen
receptor on T and B cells, and the immunoglobulin Fc region receptors on T
cells.
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