PROTECTIVE AND CELLULAR IMMUNE RESPONSES TO IDIOTYPIC DETERMINANTS ON CELLS FROM A SPONTANEOUS LYMPHOMA OF NZB/NZW F1 MICE

doi:10.1084/jem.140.6.1547

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PROTECTIVE AND CELLULAR IMMUNE RESPONSES TO IDIOTYPIC DETERMINANTS ON CELLS FROM A SPONTANEOUS LYMPHOMA OF NZB/NZW F₁ MICE

Susumu Sugai ¹, Donald W. Palmer ¹, Norman Talal ¹, and Isaac P. Witz ¹

¹ From the Department of Medicine, University of California at San Francisco, San Francisco, California 94143 and the Clinical Immunology Section, Veterans Administration, San Francisco, California 94121

A spontaneous lymphoma (141) producing monoclonal IgM is establishedin NZB/NZW F₁ (B/W) mice who spontaneously develop an autoimmunedisease. Idiotypic determinants of 141 IgM are present on thelymphoma cell surface as shown by indirect immunofluorescenceand specific cytotoxicity with rabbit anti-idiotypic antiserum.Fluorescence and cytotoxicity are inhibited by 141 IgM but notby 104E IgM, a monoclonal IgM produced by a BALB/c plasmacytoma.

Immunizationof B/W mice with 141 IgM before transplantation of lymphoma141 confers protective immunity. No such protection occurs afterimmunization with 104E IgM or other unrelated proteins. Protectedmice contain spleen cells cytotoxic for 141 lymphoma cells.This cytotoxicity is blocked by incubation of spleen cells with141 IgM but not with 104E IgM. Moreover, splenic lymphocytesfrom protected mice are stimulated to synthesize DNA by 141IgM but not by 104E IgM. These results suggest that specificcellular immune responses to idiotypic determinants may participatein the observed protection against challenge with the correspondingB-cell tumor.

Submitted on July 18, 1974

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