The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 140, 1404-1409, Copyright © 1974 by The Rockefeller University Press

BRIEF DEFINITIVE REPORTS

A SYNTHETIC GLYCOLIPID WITH B-CELL MITOGENIC ACTIVITY

David L. Rosenstreich 1, Jean Asselineau 1, Stephan E. Mergenhagen 1, and Alois Nowotny 1

1 From the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014, and the Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

A synthetic glycolipid, N-palmitoyl-D-glucosamine, (NPG) was found to be a potent B-cell mitogen, that stimulated spleen cells from nu/nu as well as from normal mice. The proper dispersion of the water insoluble preparation is critical for the elicitation of this mitogenic effect. Limulus lysate clotting assay indicated that the NPG preparation either contains only 0.001% endotoxin contamination, or that NPG itself is 10–5 times less active in this assay than purified endotoxic LPS. Since such low levels of endotoxin concentration are not mitogenic, it is concluded that synthetic N-palmitoyl-D-glucosamine, when properly dispersed, is itself a B-cell mitogen.

Submitted on July 8, 1974


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