GENETIC CONTROL OF IMMUNE RESPONSES IN VITRO: V. STIMULATION OF SUPPRESSOR T CELLS IN NONRESPONDER MICE BY THE TERPOLYMER L-GLUTAMIC ACID60-L-ALANINE30-L-TYROSINE10 (GAT)

doi:10.1084/jem.140.3.648

This Article

	Full Text (PDF, 682K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Kapp, J. A.
	Articles by Benacerraf, B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Kapp, J. A.
	Articles by Benacerraf, B.


Social Bookmarking

	What's this?

ARTICLE

GENETIC CONTROL OF IMMUNE RESPONSES IN VITRO : V. STIMULATION OF SUPPRESSOR T CELLS IN NONRESPONDER MICE BY THE TERPOLYMER L-GLUTAMIC ACID⁶⁰-L-ALANINE³⁰-L-TYROSINE¹⁰ (GAT)

Judith A. Kapp ¹, Carl W. Pierce ¹, Stuart Schlossman ¹, and Baruj Benacerraf ¹

¹ From the Departments of Pathology and Medicine, Harvard Medical School, Boston, Massachusetts 02115

In recent studies we have found that GAT not only fails toelicit a GAT-specific response in nonresponder mice but alsospecifically decreases the ability of nonresponder mice to developa GAT-specific PFC response to a subsequent challenge with GATbound to the immunogenic carrier, MBSA. Studies presented inthis paper demonstrate that B cells from nonresponder, DBA/1mice rendered unresponsive by GAT in vivo can respond in vitroto GAT-MBSA if exogenous, carrier-primed T cells are added tothe cultures. The unresponsiveness was shown to be the resultof impaired carrier-specific helper T-cell function in the spleencells of GAT-primed mice.

Spleen cells from GAT-primed micespecifically suppressed the GAT-specific PFC response of spleencells from normal DBA/1 mice incubated with GAT-MBSA. This suppressionwas prevented by pretreatment of GAT-primed spleen cells withanti- $theta$ serum plus C or X irradiation. Identification of the suppressorcells as T cells was confirmed by the demonstration that suppressorcells were confined to the fraction of the column-purified lymphocyteswhich contained $theta$ -positive cells and a few non-Ig-bearing cells.The significance of these data to our understanding of Ir-generegulation of the immune response is discussed.

Submitted on May 23, 1974

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Nicholson, L. B., Murtaza, A., Hafler, B. P., Sette, A., Kuchroo, V. K. (1997). A T cell receptor antagonist peptide induces T cells that mediate bystander suppression and prevent autoimmune encephalomyelitis induced with multiple myelin�antigens. Proc. Natl. Acad. Sci. USA 94: 9279-9284 [Abstract] [Full Text]
Berzofsky, J. (1985). Intrinsic and extrinsic factors in protein antigenic structure. Science 229: 932-940 [Abstract]
Benacerraf, B (1981). Role of MHC gene products in immune regulation. Science 212: 1229-1238
Cantor, H., Boyse, E. A. (1977). Regulation of Cellular and Humoral Immune Responses by T-cell Subclasses. Cold Spring Harb Symp Quant Biol 41: 23-32 [Abstract]
Basten, A., Loblay, R., Chia, E., Callard, R., Pritchard-Briscoe, H. (1977). Suppressor T Cells in Tolerance to Non-self and Self Antigens. Cold Spring Harb Symp Quant Biol 41: 93-103 [Abstract]
Benacerraf, B., Dorf, M. E. (1977). Genetic Control of Specific Immune Responses and Immune Suppressions by I-region Genes. Cold Spring Harb Symp Quant Biol 41: 465-475 [Abstract]
Murphy, D. B., Okumura, K., Herzenberg, L. A., Herzenberg, L. A., McDevitt, H. O. (1977). Selective Expression of Separate I-region Loci in Functionally Different Lymphocyte Subpopulations. Cold Spring Harb Symp Quant Biol 41: 497-504 [Abstract]