INTERACTIONS OF C-REACTIVE PROTEIN WITH THE COMPLEMENT SYSTEM: I. PROTAMINE-INDUCED CONSUMPTION OF COMPLEMENT IN ACUTE PHASE SERA

doi:10.1084/jem.140.3.631

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INTERACTIONS OF C-REACTIVE PROTEIN WITH THE COMPLEMENT SYSTEM : I. PROTAMINE-INDUCED CONSUMPTION OF COMPLEMENT IN ACUTE PHASE SERA

Joan Siegel ¹, Rosemarie Rent ¹, and Henry Gewurz ¹

¹ From the Department of Immunology, Rush Medical College, Chicago, Illinois 60612

Protamine sulfate was found to consume large amounts of C selectivelyduring preincubation with sera of individuals in the "acutephase". Marked depletion of C1, C4, and C2 with minimal, ifany, depletion of C3-9, was observed. The consumption was timeand temperature dependent, occurring most rapidly and extensivelyat 37°C, 0.10 M relative salt concentration and pH 7.5–8.0;it required calcium ions. It was mediated by a heat-stable nondialyzablefactor which separated with C-reactive protein (CRP) duringfractionation and purification, correlated with serum CRP levels,and, like other known reactivities of CRP, was inhibited byphosphoryl choline. Preparations of CRP purified either fromserum or ascites resulted in consumption of large amounts ofC1, C4, and C2 when preincubated with normal serum and protamine.We conclude that CRP is a potent activator of the C system atthe level of C1, and that polycations such as protamine sulfateare substrates of CRP which can bring about this activation.It seems not unlikely that one role of CRP in health and diseaseinvolves its ability to interact with the C system.

Submitted on April 8, 1974

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