UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES

doi:10.1084/jem.139.5.1084

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ARTICLE

UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES

Robert H. Bigley ¹ and Libuse Stankova ¹

¹ From the Division of Medical Genetics, University of Oregon Medical School, Portland, Oregon 97201

Incubation of human leukocytes with dehydroascorbate (DHA)results in an increase in their reduced ascorbate (AA) contentand hexose monophosphate shunt (HMS) activity, independent ofoxygen supply. Incubation with AA induces these changes onlyin the presence of oxygen. The increase in HMS activity observedas cell AA increases by 1 µmol is the same during incubationwith either DHA or AA.

We propose that human leukocytes takeup ascorbate as DHA (AA after oxidation to DHA) and reduce itpromptly to AA, and that HMS stimulation upon incubation witheither AA or DHA is a result of DHA reduction.

Submitted on February 4, 1974

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