GENETICS OF A NEW IgVH (T15 IDIOTYPE) MARKER IN THE MOUSE REGULATING NATURAL ANTIBODY TO PHOSPHORYLCHOLINE

doi:10.1084/jem.139.4.983

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ARTICLE

GENETICS OF A NEW IgV_H (T15 IDIOTYPE) MARKER IN THE MOUSE REGULATING NATURAL ANTIBODY TO PHOSPHORYLCHOLINE

R. Lieberman ¹, M. Potter ¹, E. B. Mushinski ¹, W. Humphrey Jr. ¹, and S. Rudikoff ¹

¹ From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

The idiotype present on the Fab of a phosphorylcholine-bindingIgA myeloma protein TEPC 15 (T15) of BALB/c origin was foundin normal serum of BALB/c mice. Molecules carrying the T15 idiotypein normal serum could be adsorbed with Sepharose phosphorylcholinebeads and R36A pneumococci. The T15 idiotype is absent in germ-freeBALB/c but appears when the mice are conventionalized. A surveyof normal sera of inbred strains for the T15 idiotype showedit to be present in BALB/c, 129, C57L, C58, and ST and absentor in low levels in CBA, C3H, C57BL/6, C57BL/Ka, C57BL/10, SJL,B10.D2, DBA/2, RIII, A, AL, AKR, NZB, and NH inbred strainsof mice. The T15 idiotype is associated with some but not allstrains carrying the IgC_H allotypes found in BALB/c. Linkageof genes controlling the T15 idiotype in normal serum to theIgC_H locus of BALB/c was demonstrated in F₂ progeny of a BALB/cand C57BL cross, Bailey's recombinant inbred strains, C x BD,C x BE, C x BG, C x BH, C x BI, C x BJ, C x BK, and CB20 congenicstrains. Among these strains, only those possessing the IgC_Hlocus of BALB/c including the F₂ progeny consisting of BALB/chomozygotes and BALB/c/C57BL heterozygotes and C x BG and Cx BJ recombinants showed the T15 idiotype.

Submitted on January 15, 1974

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