The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
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The Journal of Experimental Medicine, Vol 139, 1002-1012, Copyright © 1974 by The Rockefeller University Press

ARTICLE

BINDING OF AGGREGATED gamma-GLOBULIN TO ACTIVATED T LYMPHOCYTES IN THE GUINEA PIG

John A. van Boxel 1 and David L. Rosenstreich 1

1 From the Arthritis and Rheumatism Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases and the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014

Heat-aggregated guinea pig gamma-globulin was shown to bind to the surface membrane of a subclass of guinea pig T lymphocytes. Cells of this subpopulation were identified as T lymphocytes because these cells did not stain for surface Ig (a B-cell marker) but did form spontaneous E-rosettes with rabbit erythrocytes (a T-cell marker). A strikingly high proportion of such aggregate-binding (Agg+), E-rosette-forming (E-rosette+), but surface Ig-negative (Ig-) cells were found in an inflammatory exudate. Thus purified peritoneal exudate lymphocytes (PELs) are known to consist of over 90% T cells, and 59% of these cells bound aggregates. 10% of these Agg+ Ig- E-rosette+ cells were found in draining lymph node cell populations and none in thymus cell populations.

The high frequency amongst PELs suggested that these Aggregate+ Ig- E-rosette+ cells might be activated T cells as these are known to occur in high proportion in PEL populations. Confirmatory evidence for this postulate was provided by the striking increase (from 10% to 46%) of Ig- E-rosette+ cells that bound aggregates when lymph node cells were activated by antigen stimulation in vitro.

 Submitted on December 26, 1973


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