The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 139, 754-760, Copyright © 1974 by The Rockefeller University Press


ARTICLE

AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO

T. Hünig 1, A. Schimpl 1, and E. Wecker 1

1 From the Institute of Virology, Würzburg, West Germany

A rapid and reliable autoradiographic technique for plaque-forming cells (PFCs) using 14C rather than tritiated thymidine is described. Its application to PFCs developing in vitro shows that (a) practically all PFCs derive from precursors dividing steadily during the culture period, (b) PFC precursors divide in the absence of T-cell helper function, and (c) at least some PFCs may continue to divide.

Submitted on November 20, 1973


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