The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 1041K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hunt, S. V.
Right arrow Articles by Williams, A. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hunt, S. V.
Right arrow Articles by Williams, A. F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
The Journal of Experimental Medicine, Vol 139, 479-496, Copyright © 1974 by The Rockefeller University Press

ARTICLE

THE ORIGIN OF CELL SURFACE IMMUNOGLOBULIN OF MARROW-DERIVED AND THYMUS-DERIVED LYMPHOCYTES OF THE RAT

Simon V. Hunt 1 and Alan F. Williams 1

1 From the Medical Research Council Cellular Immunology Unit of the Sir William Dunn School of Pathology, and the Medical Research Council Immunochemistry Unit of the Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, England

The origin of immunoglobulin on the surface of TDL in the rat has been studied by comparing the binding of purified alloantibodies recognizing the Ig-1a allotype of rat light chain, with that of rabbit antirat Fab antibodies. Both reagents labeled all TDL from rats of the DA strain (Ig-1a) with two categories of cells being detected; one binding 100–2,000 molecules of antibody, the other 10,000–100,000 molecules. These categories were likely to be synonomous with T and B cells, respectively. The [125I]antiallotype antibodies did not bind to TDL from rats of the PVG/c strain (Ig-1b).

When the binding to TDL from (PVG/c x DA)F1 animals was studied it was found that allelic exclusion occurred in the heavily labeled cells, but not in the lightly labeled ones. Furthermore, when lymphocytes of one allotype were transferred to irradiated recipients of the opposite allotype and recovered from the TDL or spleen of the recipient 20–30 h later, the immunoglobulin on heavily labeled cells was of the donor type, while that of lightly labeled ones bore the recipient marker. Thus heavily labeled cells (B lymphocytes) had synthesized their own immunoglobulin while lightly labeled cells (T lymphocytes) had acquired theirs passively by adsorption. The class of immunoglobulin on lightly labeled cells was also studied and it was found that [125I]anti-IgM antibodies bound to an extent approaching the [125I]anti-Fab binding, while [125I]anti-IgG2a+2b antibodies gave much less binding.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS