SURFACE MARKERS ON HUMAN B AND T LYMPHOCYTES: II. PRESENCE OF EPSTEIN-BARR VIRUS RECEPTORS ON B LYMPHOCYTES

doi:10.1084/jem.138.6.1365

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SURFACE MARKERS ON HUMAN B AND T LYMPHOCYTES : II. PRESENCE OF EPSTEIN-BARR VIRUS RECEPTORS ON B LYMPHOCYTES

Mikael Jondal ¹ and George Klein ¹

¹ From the Department of Tumor Biology, Karolinska Institutet, S 104 01 Stockholm 60, Sweden

Human peripheral lymphocytes were investigated for receptorsbinding Epstein-Barr virus (EBV) because of the regular associationof this virus with infectious mononucleosis and Burkitt's lymphoma.

Thiswas done by a cytoadherence technique where virus-producingcells, displaying fresh viral determinants in their cytoplasmaticmembrane, were mixed with lymphocytes. Unfractionated lymphocyteswere found to adhere to these cells in contrast to column-purifiedT lymphocytes. The specificity of the binding was confirmedby blocking experiments that showed that sera containing hightiters of antibodies directed against the virus could partiallyinhibit the adherence in contrast to low-titer sera. It is concludedthat B lymphocytes, in contrast to T lymphocytes, have receptorsfor EBV.

In a second line of experiments it was found that establishedhuman lymphoblastoid lines that carry the EBV genome had receptorscharacteristic for B lymphocytes and did not form T-lymphocyterosettes. In contrast, a line of known T-lymphocyte origin thatdid not carry the EBV genome had receptors characteristic forT lymphocytes.

EBV-transformed simian lymphoblastoid lines hadsurface markers indicating a B-lymphocyte origin in contrastto HVS-transformed simian lines that lacked surface immunoglobulinbut carried receptors for sheep red blood cells.

Submitted on July 9, 1973

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