The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 138, 1270-1275, Copyright © 1973 by The Rockefeller University Press

BRIEF DEFINITIVE REPORTS

A NEW SURFACE MARKER ON T LYMPHOCYTES OF HUMAN PERIPHERAL BLOOD

Sten Hammarström 1, Ulla Hellström 1, Peter Perlmann 1, and Marie-Louis Dillner 1

1 From the Department of Immunology, The Wenner-Gren Institute, University of Stockholm, S-113 45 Stockholm, Sweden

Neuraminidase treatment of human peripheral blood lymphocytes uncovers cell surface receptors that bind purified A hemagglutinin from the snail Helix pomatia. No hemagglutinin was bound to untreated lymphocytes. Binding studies with 125I-labeled hemagglutinin suggested that the number of receptors on neuraminidase-treated lymphocytes was approximately 1·106/cell. The apparent association constant for hemagglutinin binding to lymphocytes, as calculated from Scatchard's plots, was 5–7·108 liters/mol.

Immunofluorescent staining with FITC-conjugated hemagglutinin gave positive reactions with approximately 60% of the lymphocytes from normal donors. Positive staining was inversely related to the number of lymphocytes with Fc or complement receptors or with surface immunoglobulin, thus suggesting that

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the lymphocytes with receptors for Helix pomatia A hemagglutinin are T cells. Cell fractionation on columns charged with hemagglutinin indicate that these receptors may be used for separating subpopulations of human peripheral lymphocytes.

 Submitted on July 5, 1973


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