RELEASE OF ENDOTOXIN IN THE FORM OF CELL WALL BLEBS DURING IN VITRO GROWTH OF NEISSERIA MENINGITIDIS

doi:10.1084/jem.138.5.1156

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RELEASE OF ENDOTOXIN IN THE FORM OF CELL WALL BLEBS DURING IN VITRO GROWTH OF NEISSERIA MENINGITIDIS

I. W. Devoe ¹ and J. E. Gilchrist ¹

¹ From the Department of Microbiology, Macdonald Campus of McGill University, Macdonald College 800, Quebec, Canada

Multiple cell wall blebs were observed on the surface of threestrains of N. meningitidis taken from log phase cultures. Theblebs originated as evaginations of the outer layer of the cellwall. Bleb production was noted on both defined or complex mediaeither as broth or a solid medium. The addition of 10% normalbovine serum to the various media did not affect the productionand release of these surface blebs. However, as broth culturesprogressed into the stationary phase of growth, the blebs disappearedfrom the surface of the cells. Blebs were present in substantialquantities in culture supernatant fluids and on cell surfacesand were readily isolated by ultracentrifugation. Analysis for2-keto-3-deoxyoctonate in cultures revealed that 18% of thetotal endotoxin of log phase cultures was present in blebs fromthe cell wall.

Submitted on July 25, 1973

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