ARTICLE

SIX BALB/c IgA MYELOMA PROTEINS THAT BIND ß-(1 6)-D-GALACTAN

Stuart Rudikoff ¹, Elizabeth B. Mushinski ¹, Michael Potter ¹, C. P. J. Glaudemans ¹, and Michael E. Jolley ¹

¹ From the National Cancer Institute, and the National Institute of Arthritis, Metabolic and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20014

Six IgA myeloma proteins of BALB/c origin which bind antigens containing ß-(1 6)-D-galactan side chains have been isolated by affinity chromatography on galactoside-BSA-Sepharose columns. Partial amino acid sequences of of the light chains to residue Cys23 and the heavy chains to reside 30 were determined on the automated sequencer. No differences were found among the six V_K sequences. Among some 50 partial V_K sequences that have thus far been determined these six chains are the only ones thus far identified in this subgroup; at least 25 V_K subgroups in the mouse have been identified so far. The heavy chain partial sequences were also very closely related but two differences were found. One protein differed from the other five by having isoleucine instead of leucine at position 5, a second protein differed from the others by having an unidentified amino acid at position 19. Using the highly sensitive inhibition of hemagglutination method it was found that each of the proteins possessed a unique inidividual antigenic determinant.

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This article has been cited by other articles:

• Potter, M., Rudikoff, S., Vrana, M., Rao, D. N., Mushinski, E. B. (1977). Primary Structural Differences in Myeloma Proteins That Bind the Same Haptens. Cold Spring Harb Symp Quant Biol 41: 661-666 [Abstract]  
• Geckeler, W., Blomberg, B., de Preval, C., Cohn, M. (1977). On the Genetic Dissection of a Specific Humoral Immune Response to {alpha}(1,3) Dextran. Cold Spring Harb Symp Quant Biol 41: 743-748 [Abstract]  
• Hood, L., Loh, E., Hubert, J., Barstad, P., Eaton, B., Early, P., Fuhrman, J., Johnson, N., Kronenberg, M., Schilling, J. (1977). The Structure and Genetics of Mouse Immunoglobulins: An Analysis of NZB Myeloma Proteins and Sets of BALB/c Myeloma Proteins Binding Particular Haptens. Cold Spring Harb Symp Quant Biol 41: 817-836 [Abstract]  

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