The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
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The Journal of Experimental Medicine, Vol 138, 847-857, Copyright © 1973 by The Rockefeller University Press

ARTICLE

SYNTHESIS, INTRACELLULAR DISTRIBUTION, AND SECRETION OF IMMUNOGLOBULIN AND H-2 ANTIGEN IN MURINE SPLENOCYTES

Dorothee Wernet 1, Ellen S. Vitetta 1, Jonathan W. Uhr 1, and Edward A. Boyse 1

1 From Irvington House Institute and the Department of Microbiology, New York University School of Medicine, New York 10016, the Department of Microbiology, University of Texas Southwestern Medical School, Dallas, Texas 75235, and the Division of Immunology, Sloan-Kettering Institute for Cancer Research, New York 10021

A/J spleen cells were labeled with [3H]leucine and at intervals thereafter were homogenized and separated into microsomes and cell sap. Ig and H-2 antigens were assayed in the cell fractions and cell supernatants using immunoprecipitation. In addition, cells labeled by enzymatic radioiodination were incubated to determine the rates of release of Ig and H-2 antigens from the surface. The results indicate that the majority of Ig and H-2 antigens remain membrane bound throughout their intracellular life. In contrast to Ig, H-2 antigens are neither secreted nor shed from the cell surface. It is suggested that Ig is a peripheral protein of the cell membrane, whereas H-2 antigens are integral ones. The release of Ig on a fragment of plasma membrane could occur at fixed cell surface areas that contain no H-2 antigens or from which they have migrated before release.

Submitted on April 12, 1973


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