The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 818K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Braun, D. G.
Right arrow Articles by Cramer, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Braun, D. G.
Right arrow Articles by Cramer, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
The Journal of Experimental Medicine, Vol 138, 645-658, Copyright © 1973 by The Rockefeller University Press

ARTICLE

A RABBIT FAMILY OF RESTRICTED HIGH RESPONDERS TO THE STREPTOCOCCAL GROUP A-VARIANT POLYSACCHARIDE : SELECTIVE BREEDING NARROWS THE ISOELECTRIC FOCUSING SPECTRA OF DOMINANT CLONES



Dietmar G. Braun 1, Ebbe Kjems 1, and Matthias Cramer 1

1 From the Basel Institute for Immunology, Basel, Switzerland, and the Statens Serum Institute, Copenhagen, Denmark

Immunization of rabbits from a closed colony with streptococcal Group A-variant vaccines identified about two-thirds of them as low and heterogeneous responders. One-third of the rabbits showed a restriction of the response independent from the magnitude. Selective breeding from one monoclonal high-responder male and two restricted high-responder female rabbits succeeded in segregation of high-responder progeny after two generations. Their antibody levels were on the average 2.5 times higher than those of the random group of rabbits and a small group of low-responder offspring.

Immunization of 13 offspring originating from rabbits bred for restricted high response to the streptococcal Group C polysaccharide revealed that 11 progeny were restricted high responders and 2 progeny monoclonal high responders. This finding suggests that high responsiveness to the Groups A-variant and C polysaccharides is inherited as genetically linked traits.

Selective breeding combinations between restricted and monoclonal high-responder rabbits by brother-sister matings succeeded in narrowing the isoelectric focusing spectra of Group A-variant-specific antibodies in the offspring. It furthermore revealed a preferential expression of monoclonal antibodies after three generations with a similar net charge as those identified first in the original monoclonal paternal parent. These data suggest that similar copies of structural genes for the variable regions are transmitted from the parent to the progeny.

 Submitted on May 23, 1973


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS