The Journal of Experimental Medicine
Aegean Conferences: 2009 Conferences
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The Journal of Experimental Medicine, Vol 138, 495-507, Copyright © 1973 by The Rockefeller University Press

ARTICLE

COMPLEMENT-DEPENDENT RELEASE OF IMMUNE COMPLEXES FROM THE LYMPHOCYTE MEMBRANE

Gary W. Miller 1, Paul H. Saluk 1, and Victor Nussenzweig 1

1 From the Department of Pathology, New York University Medical Center, New York 10016

Soluble antigen-antibody-complement complexes bound to mouse B lymphocytes are rapidly released from the cell membrane in the presence of normal serum from several mammalian species. The release is not the result of antigen-antibody dissociation or extensive degradation of the complexes. However, the released complexes have been altered because they will no longer bind to fresh lymphocytes. The release is not the result of lymphocyte damage mediated by complement. It is complement-dependent, and is generated either preferentially or exclusively via the alternate pathway, since it occurs in C4-deficient serum, is Mg++ but not Ca++ dependent, and requires C3 proactivator. C3 inactivator is not involved. The release activity of the serum, once generated, is unstable at 37°C. The release of complexes from the lymphocyte membrane by serum provides a convenient assay for the functioning of the alternate pathway in the mouse and in other species.

Submitted on May 16, 1973


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