IMMUNOLOGIC STUDIES OF THE MAJOR NONIMMUNOGLOBULIN PROTEIN OF AMYLOID: I. IDENTIFICATION AND PARTIAL CHARACTERIZATION OF A RELATED SERUM COMPONENT

doi:10.1084/jem.138.2.373

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IMMUNOLOGIC STUDIES OF THE MAJOR NONIMMUNOGLOBULIN PROTEIN OF AMYLOID : I. IDENTIFICATION AND PARTIAL CHARACTERIZATION OF A RELATED SERUM COMPONENT

Mark Levin ¹, Mordechai Pras ¹, and Edward C. Franklin ¹

¹ From the Irvington House Institute, Rheumatic Diseases Study Group and the Department of Medicine, New York University Medical Center, New York 10016

Antisera have been prepared against the major nonimmunoglobulincomponent of secondary and familial Mediterranean fever (FMF)associated amyloid which has been called A component or acidsoluble fraction (ASF). The antisera were shown to be monospecificfor ASF by precipitation of ¹²⁵I-labeled antigen and gave areaction of identity with four different ASF preparations. Theantisera were able to detect a circulating component in humanserum that migrated in the $alpha$ 1-globulin region. This circulatingcomponent gave a line of identity with degraded ASF by doubleimmunodiffusion. 57 normal sera and 89 sera from patients withdiseases known to be frequently associated with amyloidosiswere tested by immunodiffusion for the circulating ASF component.7% of normal sera and 50–80% of the pathologic sera hadelevated amounts of this component. Absorption studies showedthat all normal sera probably have small amounts of this componentwhile cord sera do not have detectable amounts. This componentwas partially purified and was shown to be slightly larger thanalbumin. The relation of the circulating component to the acidsoluble fraction of amyloid is discussed.

Submitted on March 18, 1973

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