ARTICLE

¹ From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

The role of C3 proactivator (C3PA) and a factor isolated from cobra venom (CoF) in the formation of a principle able to cleave C3 was investigated. The results clearly demonstrate two modes of interaction of C3PA with CoF. In isolated form, C3PA and CoF were found to form a reversible protein-protein complex in free solution. This complex had some C3 cleaving activity. In the presence of minute amounts of a partially purified normal serum substance, factor D, the C3PA-CoF complex was stabilized and its efficiency in cleaving C3 was greatly increased. Factor D is thus an activator of C3PA. A firm complex composed of C3PA and CoF, and possessing C3 cleaving activity, was also formed by addition of CoF to serum.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Harris, C. L., Abbott, R. J. M., Smith, R. A., Morgan, B. P., Lea, S. M. (2005). Molecular Dissection of Interactions between Components of the Alternative Pathway of Complement and Decay Accelerating Factor (CD55). J. Biol. Chem. 280: 2569-2578 [Abstract] [Full Text]  
• Rawal, N., Pangburn, M. K. (2000). Functional Role of the Noncatalytic Subunit of Complement C5 Convertase. J. Immunol. 164: 1379-1385 [Abstract] [Full Text]  
• Alper, C., Balavitch, D (1976). Cobra venom factor: evidence for its being altered cobra C3 (the third component of complement). Science 191: 1275-1276 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS