The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 832K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kunkel, H. G.
Right arrow Articles by Capra, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kunkel, H. G.
Right arrow Articles by Capra, J. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
The Journal of Experimental Medicine, Vol 137, 331-342, Copyright © 1973 by The Rockefeller University Press

ARTICLE

CROSS-IDIOTYPIC SPECIFICITY AMONG MONOCLONAL IGM PROTEINS WITH ANTI-gamma-GLOBULIN ACTIVITY

H. G. Kunkel 1, V. Agnello 1, F. G. Joslin 1, R. J. Winchester 1, and J. D. Capra 1

1 From The Rockefeller University, New York 10021, and the Department of Microbiology, Mount Sinai School of Medicine, City University of New York, New York 10029

Through the use of absorbed idiotypic antisera prepared against single isolated monoclonal IgM anti-gamma-globulins, partial cross-idiotypic specificity was demonstrated with other IgM anti-gamma-globulins. Such antisera classified these proteins into at least three groups. The major group which included 60% of the anti-gamma-globulins was particularly homogeneous. The anti-gamma-globulin specific antigens were detected best in hemagglutination and hemagglutination inhibition systems. They were not found in monoclonal IgM proteins that lacked anti-gamma-globulin activity although related antigens were detected at low concentrations in pooled immunoglobulin preparations as well as in heterogeneous anti-Rh antibodies.

Several lines of evidence were obtained indicating that the antibody combining site was involved in the specific determinants. Attempts were made to analyze the fine specificity of each anti-gamma-globulin for the Fc fragment of different subclasses of human immunoglobulins as well as those of other species. Differences were observed but these were not readily related to the cross-specificity antigens.

The anti-gamma-globulin specific antigens were very analogous to those previously described for monoclonal IgM cold agglutinins. Although each protein could be distinguished from all the others on the basis of individual idiotypic antigens, the antigens common to the specific groups of proteins with each of these activities were prominent and readily detected with multiple antisera. The results indicate basic similarities between proteins of a given activity even in unrelated individuals.

 Submitted on October 26, 1972


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS