GRANULATION TISSUE AS A CONTRACTILE ORGAN: A STUDY OF STRUCTURE AND FUNCTION

doi:10.1084/jem.135.4.719

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ARTICLE

GRANULATION TISSUE AS A CONTRACTILE ORGAN : A STUDY OF STRUCTURE AND FUNCTION

G. Gabbiani ¹, B. J. Hirschel ¹, G. B. Ryan ¹, P. R. Statkov ¹, and G. Majno ¹

¹ From the Department of Pathology, University of Geneva, Geneva, Switzerland

Contracting granulation tissues contain fibroblasts that developcharacteristics typical of smooth muscle: (a) They contain anextensive cytoplasmic fibrillar system. (b) They show immunofluorescentlabeling of their cytoplasm with human anti-smooth muscle serum.(c) The nuclei show complicated folds and indentations, indicativeof cellular contraction. (d) There are cell-to-cell and cell-to-stromaattachments. (e) It is possible to extract similar quantitiesof actomyosin (having the same adenosine triphosphatase activity)from granulation tissue and from pregnant rat uterus. (f) Stripsof granulation tissue, when tested pharmacologically in vitro,behave similarly to smooth muscle.

All these data support theview that, under certain conditions, fibroblasts can differentiateinto a cell type structurally and functionally similar to smoothmuscle and that this cell, the "myo-fibroblast," plays an importantrole in connective tissue contraction.

Submitted on October 31, 1971

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