The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
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The Journal of Experimental Medicine, Vol 134, 1403-1416, Copyright © 1971 by The Rockefeller University Press

ARTICLE

ELECTRON MICROSCOPIC LOCALIZATION OF IMMUNOGLOBULIN E ON THE SURFACE MEMBRANE OF HUMAN BASOPHILS

Albert L. Sullivan M.D.1, Philip M. Grimley M.D.1, and Henry Metzger M.D.1

1 From the Arthritis and Rheumatism Branch, National Institute of Arthritis and Metabolic Diseases, and the Ultrastructural Pathology Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

We have examined human leukocyte preparations for the presence of surface-bound IgE by electron microscopy. Basophil-enriched leukocytes were reacted with burro anti-IgE, a hybrid antibody to burro IgG and ferritin, and ferritin, with or without prior incubation of the cells with an IgE myeloma protein.

In the absence of preincubation with IgE small amounts of ferritin were fixed to the surface of basophils but on no other cells. When cells were preincubated with IgE the amount of ferritin fixation on the basophils was markedly increased and a small amount of ferritin was also bound to platelets but again to no other leukocytes. The distribution of ferritin on the basophil surface appeared dependent upon the temperature at which the cells were kept during and after reaction with the various reagents. Basophil sections from cells kept at 0°C had ferritin bound to the surface membrane in patches distributed around the entire circumference. Basophil sections from cells prepared at room temperature had ferritin distributed assymetrically covering a surface membrane segment one-fifth to one-half of the circumference.

In control studies in which monomeric IgG was substituted for the IgE and burro anti-IgG was used instead of burro anti-IgE, no cellular fixation of ferritin was observed.

 Submitted on July 19, 1971


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