HUMAN IMMUNITY TO THE MENINGOCOCCUS: III. PREPARATION AND IMMUNOCHEMICAL PROPERTIES OF THE GROUP A, GROUP B, AND GROUP C MENINGOCOCCAL POLYSACCHARIDES

doi:10.1084/jem.129.6.1349

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HUMAN IMMUNITY TO THE MENINGOCOCCUS : III. PREPARATION AND IMMUNOCHEMICAL PROPERTIES OF THE GROUP A, GROUP B, AND GROUP C MENINGOCOCCAL POLYSACCHARIDES

Emil C. Gotschlich M.D.¹, Teh Yung Liu Ph. D.¹, and Malcolm S. Artenstein M.D.¹

¹ From The Department of Bacteriology, Walter Reed Army Institute of Research, Washington, D. C. 20012, and The Department of Biology, Brookhaven National Laboratories, Brookhaven, New York 11719

The group-specific polysaccharides of group A and group C meningococcihave been isolated by a new procedure which employs the cationicdetergent Cetavlon to precipitate these polysaccharides fromthe whole culture. The A and C polysaccharide prepared by thismethod are noteworthy because they are of high molecular weight.The main constituent of the A polysaccharide is N-acetyl, O-acetylmannosamine phosphate; of the C polysaccharide N-acetyl, O-acetylneuraminic acid. This purification procedure, when applied tocultures of group B organisms, yields a polysaccharide consistingprimarily of N-acetyl neuraminic acid.

A passive hemagglutinationtest developed to measure antibodies to the polysaccharidesdemonstrated the specificity of these antigens. Using a hemagglutinationinhibition test, these antigens were again found to be group-specific,and this test could be used for serogrouping meningococcal isolates.

Submitted on February 19, 1969

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