THE RENAL HANDLING OF HEMOGLOBIN: I. GLOMERULAR FILTRATION

doi:10.1084/jem.129.5.909

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ARTICLE

THE RENAL HANDLING OF HEMOGLOBIN : I. GLOMERULAR FILTRATION

H. Franklin Bunn M.D.¹, William T. Esham ¹, and Robert W. Bull D.V.M.¹

¹ From the Blood Transfusion and Pathology Divisions, United States Army Medical Research Laboratory, Fort Knox, Kentucky 40121

The glomerular filtration of hemoglobin ( $alpha$ ₂ß₂) was studiedunder conditions in which its dissociation into $alpha$ ß dimerswas experimentally altered. Rats receiving hemoglobin treatedwith the sulfhydryl reagent bis(N-maleimidomethyl) ether (BME)showed a much lower renal excretion and prolonged plasma survivalas compared with animals injected with untreated hemoglobin.Plasma disappearance was also prolonged in dogs receiving BMEhemoglobin. Gel filtration data indicated that under physiologicalconditions, BME hemoglobin had impaired subunit dissociation.In addition, BME hemoglobin showed a very high oxygen affinityand a decreased rate of auto-oxidation.

Glomerular filtrationwas enhanced under conditions which favor the dissociation ofhemoglobin into dimers. Cat hemoglobin, which forms subunitsmuch more extensively than canine hemoglobin, was excreted morereadily by the rat kidney. The renal uptake of ⁵⁹Fe hemoglobininjected intra-arterially into rabbits varied inversely withthe concentration of the injected dose.

Submitted on January 5, 1969

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