The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 128, 223-233, Copyright © 1968 by The Rockefeller University Press

ARTICLE

STUDIES ON THE COMPETENCE OF SINGLE CELLS TO PRODUCE ANTIBODIES OF TWO SPECIFICITIES

Harriet Gershon 1, Sara Bauminger Ph.D.1, Michael Sela Ph.D.1, and Michael Feldman Ph.D.1

1 From The Weizmann Institute of Science, Rehovoth, Israel

Two series of experiments were performed, utilizing a modification of the hemolysin plaque technique which registers 19S antibody, in an attempt to determine the frequency of cells capable of simultaneously producing antibody to two non-cross-reacting antigens. Mice were immunized i.v. with rabbit and camel RBC and their spleens assayed for cells producing antibody against both antigens. 16,904 cells producing antibody of one or the other specificity, from 26 mice, were counted. Not one cell was detected which produced antibody of two specificities. Rabbits were immunized intradermally with HSA to which polyalanyl and p-azobenzenearsonate groups were chemically attached. The individual haptens, polyalanyl, and p-azobenzenearsonate groups were coupled to separate aliquots of SRBC, and the lymph nodes of immunized rabbits were assayed for cells releasing antibody against both haptens. In a study of 11 rabbits, after counting 27,845 cells producing antibody, we detected no "double" plaques.

Submitted on March 26, 1968


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