The Journal of Experimental Medicine, Vol 128, 171-187,
Copyright © 1968 by The Rockefeller University Press
RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY
:
II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE
J. D. Wakefield Ph.D.1 and
G. J. Thorbecke M.D.1
1 From the Department of Pathology, New York University School of Medicine, New York 10016
White-pulp cells and whole spleen from donor mice immunized with sheep erythrocytes were transferred intravenously to heavily irradiated mice. The numbers of plaque-forming cells and the amount of hemagglutinating antibody produced after reexposure to antigen were measured.
When reexposure to sheep erythrocytes was delayed, a much greater response occurred in the transferred cells. Peak responsiveness was reached at 24 hr after transfer. This "lag effect" was greatly reduced by repeated injections of 5-bromodeoxyuridine into the recipient mice prior to challenge with antigen. It was therefore concluded that much of the increase in responsiveness was due to a proliferation of "primed" cells after cell transfer. The fact that a significant response was given by the transferred cells in spite of 5-bromodeoxyuridine treatment suggested that some of the primed cells were nondividing.
White pulp was a much richer source of responsive cells than was whole spleen.
Submitted on February 8, 1968
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